Homework. Unanswered
Which bacteria would you not use a Gram stain on? Select all that apply.
i Multiple answers: Multiple answers are accepted for this question
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a
Archaebacteria
b
Escherichia coli
с
Staphylococcus spp. and Bacillus spp.
d
Gram-neutral bacteria (Mycobacterium spp.)
e
Gram-variable bacteria Arthrobacter spp. and actinomycetes

Gram-positive bacteria are bacteria that have thick cell walls which yield positive results in the Gram staining test. Lipoteichoic acid is a major component of the cell wall of gram-positive bacteria.

All bacteria indicated in the question can be classified by the Gram staining test:

Actinomycetes are Gram-positive bacteria
The genus Arthrobacter includes Gram-positive bacteria
Escherichia coli (E. coli) is a Gram-negative bacterium
Staphylococcus spp. are Gram-positive bacteria
Bacillus spp are Gram-positive bacteria
Mycobacterium spp. are Gram-positive bacteria

Prokaryotes can be divided into two domains: Bacteria and Archaea.

Gram staining is a method used to classify bacteria, but this method IS NOT USED to stain Archaea.

In consequence, I would not use the Gram test to stain Archaebacteria because Archaebacteria aren't bacteria (Option A is correct).

Archaebacteria belong to the Archaea domain.

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