The correct order of compounds from bottom to top while running a TLC plate is:
- Spot your compound to the TLC plate baseline.
- Place the plate into the jar. Ensure the solvent level remains below the baseline.
- Let the solvent front run till 1 cm from the top of the plate.
- Remove the plate from the jar. Mark the solvent front using a pencil.
- View the plate under UV light.
TLC is short for thin-layer chromatography, a technique for the separation of substances based on their mobility on solvents. TLC plate is a sheet of metal, glass, or plastic coated with a thin layer of a solid adsorbent.
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