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Answer :

The best solution to separate the dyes is the solution of any non polar solvent with polar solvent.

The most common two component solvent system are: Ether/petroleum ether. Ether/hexane, Ether/pentane.

The other components used to separate dyes are: water, methanol, isopropanol, formic acid which are staple reagents in fast protein liquid chromatography, high performance liquid chromatography and liquid chromatography-mass spectrometry.

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