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5. What would be the consequence of treating the vector, before ligation, with calf intestinal phosphatase?

A. It would prevent the plasmid's DNA from reversing polarity during ligation.

B. It would prevent the ends of the plasmids from being ligated.

C. It would ligate the ends of the plasmids.

D. It would reverse the polarity of plasmids' DNA

Answer :

The consequence of treating the vector, before ligation, with calf intestinal phosphatase will be B. It would prevent the ends of the plasmids from being ligated.

Calf intestinal phosphatase is a phosphatase enzyme that is derived from the intestine of the calf. The function of this enzyme is to remove the phosphatases present in the 3' and the 5' of a DNA segment by cleaving them.

Vectors, such as plasmids, are treated with the calf intestinal enzyme in order to prevent the plasmid from being ligated again. In order to add our gene of interest to the vector, the calf intestinal phosphatase is added so that the vector binds to the gene of interest rather than itself.

To learn more about plasmids, click here:

https://brainly.com/question/2088910

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