Answer :
Because the cuvette's walls can vary in breadth, this is the case. If you don't enter it exactly the same way each time, it will change how much light passes through the sample and will have an impact on your results.
The cuvette has a line that must line up with the sample holder's line because of this. hence, readings had no impact.
The cuvette's route length in the spectrophotometer should always stay the same.
This is because the radiation should have a constant path length as it goes through the sample inside the cuvette. By doing this, the cuvette through which the light will travel won't get scratched.
Scratches on the cuvette will result in inaccurate measurements of the concentration or absorbance that is being assessed, producing inaccurate findings.
Therefore, the line on the cuvette is always aligned with that of the sample holder in order to establish a uniform path length and obtain accurate findings.
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