👤
Answered

If pARA-R is digested with BamHI and HindIII, what fragments are produced? Record the nucleotide sequence of the sticky ends and the length of each fragment (bp), and indicate the genes and other important sequences present on each fragment. There are two fragments are produced. They are RFP with pBAD and Ara-C with ori with Amp-R. The RFP plus pBAD is 807 BP, and Ara-C, ori, and Amp-R is 4495 BP.

Answer :

The plasmid would be split in two if the enzymes BamHI and HindIII were used to digest pARA-R.

Restrictions enzymes BamHI and HindIII are both present. DNA is sliced by restriction enzymes at a particular base pair. The two segments are red fluorescent protein and pBAD, which are 807 base pairs (bp), and Ara-C, the ori sequence, and Amp-R, which is 4,495 bp, in the other fragment. There should be six pieces of each of the enzymes A and B and eight fragments of enzyme C. The BamHI recognition site, GGATCC, can be found five times in DNA. Eight fragments that can be used as molecular weight standards for agarose gel electrophoresis are produced when lambda DNA is digested with HindIII.

To learn more about plasmids click here https://brainly.com/question/15461017

#SPJ4

Go Teaching: Other Questions