The fourth option is correct . Ions are separated by their hydrophobicity . Since, they are separated according to mass to charge ratio.
The exit of a small-scale chromatography column can be coupled directly to the input of a mass spectrometer using nano electrospray ionization. A needle with a 10-15 um point is used to channel the flow from the column. The ions are sorted and separated based on mass-to-charge (m/z) ratios after being ionized.
The molecular weight of the particles can be ascertained using mass spectrometry (MS), an analytical technique that separates ionized particles like atoms, molecules, and clusters by using variations in the ratios of their charges to their respective masses (mass/charge; m/z).
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